The ball numbers for the first-round draw are listed under.
1. Accrington Stanley
2. AFC Wimbledon
3. Barnet
4. Barnsley
5. Barrow
6. Blackpool
7. Bolton Wanderers
8. Bradford Metropolis
9. Bristol Rovers
10. Bromley
11. Burton Albion
12. Cambridge United
13. Cardiff Metropolis
14. Cheltenham City
15. Chesterfield
16. Colchester United
17. Crawley City
18. Crewe Alexandra
19. Doncaster Rovers
20. Exeter Metropolis
21. Fleetwood City
22. Gillingham
23. Grimsby City
24. Harrogate City
25. Huddersfield City
26. Leyton Orient
27. Lincoln Metropolis
28. Luton City
29. Mansfield City
30. Milton Keynes Dons
31. Newport County
32. Northampton City
33. Notts County
34. Oldham Athletic
35. Peterborough United
36. Plymouth Argyle
37. Port Vale
38. Studying
39. Rotherham United
40. Salford Metropolis
41. Shrewsbury City
42. Stevenage
43. Stockport County
44. Swindon
45. Tranmere Rovers
46. Walsall
47. Wigan Athletic
48. Wycombe Wanderers
49. Darlington or AFC Telford United
50. Macclesfield or Stamford
51. Gainsborough Trinity or Hartlepool United
52. Carlisle United or Boston United
53. Rochdale or York Metropolis
54. Runcorn Linnets or Buxton
55. Morecambe or Chester
56. South Shields or Spalding United
57. Tamworth or Hyde United
58. Scunthorpe United or King’s Lynn City
59. Aveley or Gateshead
60. Spennymoor City or Billericay City
61. Southport or FC Halifax City
62. Altrincham or Harborough City
63. Worthing or Forest Inexperienced Rovers
64. Maldon & Tiptree or Flackwell Heath
65. Woking or Brackley City
66. Wealdstone or Whitstable City
67. Slough City or Enfield City
68. Eastbourne Borough or Boreham Wooden
69. Hampton & Richmond Borough or Eastleigh
70. Southend United or Folkestone Invicta
71. Ebbsfleet United or Solihull Moors
72. Braintree City or Farnborough
73. Tonbridge Angels or Chatham City
74. Weston Tremendous Mare or Needham Market
75. Hemel Hempstead City or Yeovil City
76. Banbury United or St Albans Metropolis
77. Chelmsford Metropolis or Chippenham City
78. AFC Totton or Truro Metropolis
79. Dorking Wanderers or Aldershot City
80. Farnham City or Sutton United
